Premium
Plant‐ and Fish‐Derived n‐3 PUFAs Suppress Citrobacter Rodentium –Induced Colonic Inflammation
Author(s) -
Määttänen Pekka,
Lurz Eberhard,
Botts Steven R.,
Wu Richard Y.,
Robinson Shaiya C.,
Yeung C. William,
Colas Romain,
Li Bo,
JohnsonHenry Kathene C.,
Surette Marc E.,
Dalli Jesmond,
Sherman Philip M.
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201900873
Subject(s) - inflammation , polyunsaturated fatty acid , citrobacter rodentium , colitis , fish oil , biology , feces , food science , microbiology and biotechnology , immunology , chemistry , biochemistry , fish <actinopterygii> , fatty acid , fishery
Scope Marine‐derived n ‐3 PUFAs may ameliorate inflammation associated with inflammatory bowel diseases. Plant‐derived n ‐3 PUFAs are thought to be inferior owing to shorter chain lengths. The aim of this study is to compare the impact of plant‐ and fish‐derived PUFAs on murine colitis. Methods and results C57BL/6 mice are fed high fat (36% kcal) diets with either 2.5% w/w sunflower oil (SO), flaxseed oil (FSO), ahiflower oil (AO), or fish oil (FO). After 4 weeks, mice are orogastrically challenged with Citrobacter rodentium (10 8 CFU) or sham gavaged. Fecal shedding is assayed at 2, 7, 10, and 14 days post infection (PI), and fecal microbiota at 14 days PI. Colonic inflammation and lipid mediators are measured. Supplementation regulates intestinal inflammation with crypt lengths being 66, 73, and 62 ±17 µm shorter (compared to SO) for FSO, AO, and FO respectively, p < 0.01. FSO blunts pathogen shedding at the peak of infection and FSO and AO both enhance fecal microbial diversity. FO attenuates levels of lipoxin and leukotriene B 4 while plant oils increase pro‐resolving mediator concentrations including D, E, and T‐series resolvins. Conclusion Plant and fish n ‐3 PUFAs attenuate colitis‐induced inflammation while exhibiting characteristic pro‐resolving lipid mediator metabolomes. Plant oils additionally promote microbial diversity.