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Lactobacillus Casei Zhang Alleviates Shrimp Tropomyosin‐Induced Food Allergy by Switching Antibody Isotypes through the NF‐κB‐Dependent Immune Tolerance
Author(s) -
Fu Linglin,
Xie Menghua,
Wang Chong,
Qian Yi,
Huang Jianjian,
Sun Zhihong,
Zhang Heping,
Wang Yanbo
Publication year - 2020
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201900496
Subject(s) - food allergy , immune system , allergy , immunology , probiotic , biology , immune tolerance , regulatory t cell , antibody , t cell , microbiology and biotechnology , il 2 receptor , bacteria , genetics
Scope Shellfish allergy is an important cause of food allergy, and tropomyosin (TM) is the major allergen within shellfish. Probiotics are safe bacteria that benefit host health and nutrition and is proposed as a novel approach for treating immunological diseases, including food allergies. Methods and results The probiotic strain Lactobacillus casei Zhang (LcZ) isolated from koumiss is investigated for its capacity to modulate food allergy induced by TM in BALB/c mice. Oral administration of LcZ attenuated allergy symptoms and intestinal epithelial damage. Furthermore, flow cytometry, real‐time quantitative PCR, and ELISA demonstrated that LcZ administration altered the development and function of dendritic cells (DCs), T cells, and B cells, finally resulting in the change of TM‐specific antibody isotypes into a tolerogenic pattern. Moreover, an in vitro spleen cell culture model reveals that LcZ directly modulates regulatory tolerogenic DC and T cell development, dependent on the activation of the nuclear factor kappa B (NF‐κB) signaling pathway. Conclusion This work indicates the ability of LcZ to alleviate TM‐induced food allergy and demonstrates the involvement of the tolerogenic immune cells and NF‐κB signaling pathway, indicating LcZ to be a potential immunomodulator and immunotherapy assistor.