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Anti‐Diabetic, Anti‐Inflammatory, and Anti‐Oxidant Effects of Naringenin in an In Vitro Human Model and an In Vivo Murine Model of Gestational Diabetes Mellitus
Author(s) -
NguyenNgo Caitlyn,
Willcox Jane C,
Lappas Martha
Publication year - 2019
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201900224
Subject(s) - naringenin , oxidative stress , endocrinology , medicine , gestational diabetes , inflammation , insulin resistance , adipose tissue , diabetes mellitus , antioxidant , chemistry , biology , gestation , pregnancy , biochemistry , flavonoid , genetics
Scope Gestational diabetes mellitus (GDM), which affects up to 20% of pregnant women, is associated with maternal peripheral insulin resistance, low‐grade inflammation, and oxidative stress. The flavonoid naringenin has potent anti‐diabetic, anti‐inflammatory, and anti‐oxidative properties; however, its effects in GDM remain unknown. The study aimed to determine the effects of naringenin on glucose metabolism, inflammation, and oxidative stress associated with GDM both in vitro and in vivo. Methods and results In vitro, human tissue samples obtained at term elective Caesarean section are stimulated with tumour necrosis factor alpha (TNF) to develop a GDM‐like environment. Naringenin treatment significantly improves TNF‐impaired glucose uptake in skeletal muscle. In placenta and visceral adipose tissue (VAT), naringenin significantly reduces expression of pro‐inflammatory cytokines and chemokines and increases antioxidant mRNA expression. Mechanistically, naringenin suppresses nuclear factor κB activation. In vivo, pregnant heterozygous db /+ mice are used to model GDM. Daily intraperitoneal injections of GDM mice with naringenin from gestational day 10–17 significantly improve glucose tolerance, reduces IL1A mRNA expression, and increases antioxidant mRNA expression in placenta, VAT, and subcutaneous adipose tissue. Conclusion Naringenin is shown to improve insulin sensitivity, inflammation, and oxidative stress associated with GDM and shows promise as a novel preventive therapeutic.