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Regulatory Roles of Pectin Oligosaccharides on Immunoglobulin Production in Healthy Mice Mediated by Gut Microbiota
Author(s) -
Zhang Shanshan,
Hu Haijuan,
He Wanying,
Muhammad Zafarullah,
Wang Lufeng,
Liu Fengxia,
Pan Siyi
Publication year - 2019
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201801363
Subject(s) - prebiotic , immune system , gut flora , antibody , immunoglobulin a , short chain fatty acid , feces , microbiology and biotechnology , bacteria , chemistry , biology , immunoglobulin g , biochemistry , food science , immunology , butyrate , fermentation , genetics
Scope The prebiotic regulation of the gut microbiota is a promising strategy to induce protective humoral and mucosal immune responses. The potential immune‐improving effects of pectin oligosaccharides (POS) in healthy mice and the potential mechanism mediated by specific intestinal bacteria are investigated. Methods and results POS is prepared using a hydrogen‐peroxide‐assisted degradation. Mice that consumed diets containing POS are tested for microbial community shifts, short‐chain fatty acids (SCFAs), and immunoglobulin (Ig) production using quantitative real‐time polymerase chain reaction, gas chromatography, and ELISA kits. Pearson's correlation analyses are performed between Ig production and specific intestinal bacteria or SCFAs. POS treatment significantly improves the growth of healthy mice. Moreover, 4‐week POS administration results in a profound change in intestinal microbial composition and a significantly higher fecal concentration of acetate, which leads to substantial increases of the levels of fecal secretory immunoglobulin A and serum IgG. Conclusions The results suggest that the inclusion of POS in a diet can increase Ig production and optimize the composition of the gut microbiota. A significant correlation is observed between changes in Ig production and specific intestinal bacteria or acetate, providing insight into the mechanism of POS as a potential immune‐enhancing supplement.