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Dietary Prevention of Colitis by Aronia Berry is Mediated Through Increased Th17 and Treg
Author(s) -
Pei Ruisong,
Martin Derek A.,
Valdez Jonathan C.,
Liu Jiyuan,
Kerby Robert L.,
Rey Federico E.,
Smyth Joan A.,
Liu Zhenhua,
Bolling Bradley W.
Publication year - 2019
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201800985
Subject(s) - colitis , adoptive cell transfer , regulatory t cell , spleen , lamina propria , berry , immunology , mesenteric lymph nodes , t cell , immune system , chemistry , medicine , biology , il 2 receptor , botany , epithelium , pathology
Scope Increased fruit consumption is associated with reduced risk of colitis. It has been investigated whether the anti‐colitic effects of the polyphenol‐rich aronia berry ( Aronia mitschurinii ‘Viking’) are mediated through Th17 and Treg. Methods and results Colitis is induced in recombinase activating gene‐1 deficient mice injected with syngeneic CD4 + CD62L + naïve T cells. Mice consume either 4.5% w/w aronia‐berry‐supplemented or a control diet concurrent with T cell transfer. The extent of colitis and immunocyte populations are evaluated at weeks 3 to 7 after transfer. Aronia consumption prevents colitic wasting and reduces colon weight/length ratios relative to the control diet at weeks 5 and 7. Compared to the control diet, aronia feeding increases Treg in mesenteric lymph node at all colitis stages. Treg and regulatory Th17 subpopulations (IL‐17A + IL‐10 + and IL‐17A + IL‐22 + ) are increased in lamina propria and spleen at week 5 in aronia‐fed mice. Aronia feeding also decreases total CD4 + cells but increases colonic Tregs. The ability of aronia to modulate colonic cytokines is associated with functional T cell IL‐10 and increased diversity of microbiota. Conclusions Aronia berry consumption inhibits adoptive transfer colitis by increasing Treg and regulatory Th17 cells. Dietary modulation of T cells is dynamic and precedes colitic wasting.