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The Anti‐Arthritis Effect of Olive‐Derived Maslinic Acid in Mice is Due to its Promotion of Tissue Formation and its Anti‐Inflammatory Effects
Author(s) -
Shimazu Kyoko,
Fukumitsu Satoshi,
Ishijima Tomoko,
Toyoda Tsudoi,
Nakai Yuji,
Abe Keiko,
Aida Kazuhiko,
Okada Shinji,
Hino Akihiro
Publication year - 2019
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201800543
Subject(s) - arthritis , inflammation , chemistry , pharmacology , endocrinology , glucocorticoid receptor , immunology , medicine , receptor , biochemistry
Scope A previous study demonstrated that intake of olive pomace extract containing maslinic acid (MA), a triterpene, effectively prevents and alleviates arthritis in animals and humans. Here, the molecular mechanisms involved in the anti‐arthritis effect of MA have been elucidated by determining gene expression changes induced by olive‐derived MA intake in collagen antibody‐induced arthritis (CAIA) mice. Methods and results Mice are divided into the untreated (CT), CAIA (CA), and CAIA administered MA (CA + MA) groups. The CA + MA mice are fed MA at a daily dose of 200 mg kg –1 of body weight from day 1. CAIA is then induced on day 8 and evaluated on day 12. Arthritis symptoms are alleviated, and the gene expression of inflammatory cytokines is reduced in the CA + MA group compared with the CA group. A DNA microarray analysis of synovial membranes reveals that MA alters the expression levels of genes related to inflammation, including glucocorticoid responses, immune responses, and the extracellular matrix. Conclusions The preventive effect of MA on arthritis is attributable to the promotion of tissue formation as well as suppression of inflammation in the synovium via inactivation of Toll‐like receptor signaling and downregulation of leukotrienes through the glucocorticoid receptor.