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Goji Berry Modulates Gut Microbiota and Alleviates Colitis in IL‐10‐Deficient Mice
Author(s) -
Kang Yifei,
Yang Guan,
Zhang Shuming,
Ross Carolyn F.,
Zhu MeiJun
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201800535
Subject(s) - lachnospiraceae , biology , roseburia , butyrate , gut flora , prebiotic , bifidobacterium , clostridium , microbiology and biotechnology , firmicutes , food science , lycium , bacteroides , bacteria , biochemistry , fermentation , lactobacillus , medicine , alternative medicine , 16s ribosomal rna , pathology , genetics
Scope This study examines the beneficial effects of Goji berry against spontaneous colitis and its prebiotic role in IL‐10‐deficient mice. Methods IL‐10‐deficient mice are assigned to a standard rodent diet (control) or a control diet supplemented with Goji (1% of dry feed weight) for 10 weeks, at which point colonic tissues and fecal contents are collected. Results Goji supplementation decreases colonic pathobiological scores and mRNA expression of Il17a and Tgfb1 , while it enhances Muc1 expression and fecal IgA content. Illumina MiSeq sequencing reveals that Goji supplementation increases Actinobacteria phylum, resulting in a bloom of Bifidobacteria in gut microbiota. Additionally, dietary Goji promotes butyrate‐producing bacteria including Lachnospiraceae‐Ruminococcaceae family and Roseburia spp. under Clostridium cluster XIVa. Furthermore, butyrate‐producers Clostridium leptum and its dominant constituent Fecalibacterium prazusnitzii are markedly increased in the Goji group. Moreover, the gene‐encoding butyryl‐coenzyme A CoA transferase, a key enzyme responsible for butyrate synthesis in butyrate‐producing bacteria, is increased sixfold in the fecal samples of Goji group associated with increased fecal butyrate content. Conclusion Data collectively show that dietary Goji results in the blooming of Bifidobacteria and butyrate‐producing bacteria. These bacteria may cross‐feed each other, conferring preventative effects against colitis in IL‐10‐deficient mice.