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Oral Immunotherapy with a Phosphorylated Hypoallergenic Allergen Ameliorates Allergic Responses More Effectively Than Intact Allergen in a Murine Model of Buckwheat Allergy
Author(s) -
Katayama Shigeru,
Yamaguchi Daiki,
Suzuki Yuta,
Athamneh Ahmad M. Al,
Mitani Takakazu,
Satoh Rie,
Teshima Reiko,
Mine Yoshinori,
Nakamura Soichiro
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201800303
Subject(s) - hypoallergenic , immunology , allergen , allergy , phosphorylation , cd11c , sensitization , food allergy , western blot , desensitization (medicine) , immunoglobulin e , chemistry , medicine , antibody , biochemistry , receptor , gene , phenotype
Scope Buckwheat is a common food allergen frequently consumed in Asian countries, with Fag e 1 and Fag e 2 being the major buckwheat allergens. The purpose of this study is to prepare an oral immunotherapy agent by attenuating these allergens via phosphorylation. The immunomodulatory effects of phosphorylated Fag e 2 (P‐Fag e 2) in a mouse model of buckwheat allergy are evaluated. Methods and results Phosphorylated Fag e 1 (P‐Fag e 1) and P‐Fag e 2 are prepared by dry‐heating in the presence of pyrophosphate. Subsequent dot‐blot analysis using serum from food‐allergic patient indicates that both proteins exhibit reduced allergenicity upon phosphorylation. Mice subjected to oral administration of P‐Fag e 2 for 6 weeks exhibit decreased specific serum IgE and increased specific IgA after Fag e 2 sensitization compared to the sham‐treated mice. Moreover, the Peyer's patches (PP) of phosphorylated antigen‐fed mice show decreased IL‐4 production and induction of T follicular helper (Tfh) cells. Increased production of IL‐6 is observed in the CD11c + cells isolated from the PPs of P‐Fag e 2‐fed mice. Conclusion These results indicate that attenuated allergens can suppress Th2‐induced allergic responses via induction of Tfh cells, which are regulated by IL‐6 secreted from dendritic cells.