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Raspberry Supplementation Improves Insulin Signaling and Promotes Brown‐Like Adipocyte Development in White Adipose Tissue of Obese Mice
Author(s) -
Xing Tong,
Kang Yifei,
Xu Xinglian,
Wang Bo,
Du Min,
Zhu MeiJun
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201701035
Subject(s) - endocrinology , medicine , white adipose tissue , adipogenesis , adipocyte , adipose tissue , prdm16 , inflammation , insulin resistance , ampk , insulin , brown adipose tissue , thermogenesis , chemistry , biology , phosphorylation , protein kinase a , biochemistry
Scope Excessive lipid accumulation in white adipose tissue (WAT) leads to chronic inflammation and metabolic dysfunction. Raspberry (RB) contains high amount of polyphenols and dietary fibers. The objective of the study is to evaluate the effects of RB supplementation on WAT morphology, inflammation, and insulin signaling in high fat diet (HFD)‐induced obese mice, and further explore the underlying mechanisms. Methods and results C57BL/6J mice are fed with a control diet or a HFD supplemented with 0 or 5% freeze dried RB for 12 weeks. RB supplementation decreases WAT hypertrophy induced by HFD and suppresses pro‐inflammatory cytokines expression and macrophage infiltration in WAT. Meanwhile, RB addition improves insulin sensitivity of HFD‐mice. Additionally, RB supplementation drives the browning of WAT (beige adipogenesis), which is associated with elevated PGC‐1α and FNDC5/irisin contents. Consistently, the content of beige adipocyte markers including UCP1, PRDM16, Cytochrome C, Cidea, and Elvol3 is enhanced in HFD‐mice, which are correlated with increased AMPK phosphorylation and Sirt1 protein contents. Conclusion Dietary RB attenuated adipocyte hypertrophy and inflammation of WAT in HFD‐mice and improves insulin sensitivity and beige adipogenesis, which is associated with increased FNDC5/irisin content and activation of AMPK/Sirt1 pathway. RB supplementation provides a promising strategy to prevent diet‐induced obesity.