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Transcriptome‐Based Analysis in Lactobacillus plantarum WCFS1 Reveals New Insights into Resveratrol Effects at System Level
Author(s) -
Reverón Inés,
PlazaVinuesa Laura,
Franch Mónica,
las Rivas Blanca,
Muñoz Rosario,
López de Felipe Félix
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700992
Subject(s) - lactobacillus plantarum , transcriptome , biology , effector , probiotic , efflux , resveratrol , gene , genome , microbiology and biotechnology , computational biology , genetics , bacteria , biochemistry , gene expression , lactic acid
Scope This study was undertaken to expand our insights into the mechanisms involved in the tolerance to resveratrol (RSV) that operate at system‐level in gut microorganisms and advance knowledge on new RSV‐responsive gene circuits. Methods and results Whole genome transcriptional profiling was used to characterize the molecular response of Lactobacillus plantarum WCFS1 to RSV. DNA repair mechanisms were induced by RSV and responses were triggered to decrease the load of copper, a metal required for RSV‐mediated DNA cleavage, and H 2 S, a genotoxic gas. To counter the effects of RSV, L. plantarum strongly up‐ or downregulated efflux systems and ABC transporters pointing to transport control of RSV across the membrane as a key mechanism for RSV tolerance. L. plantarum also downregulated tRNAs, induced chaperones, and reprogrammed its transcriptome to tightly control ammonia levels. RSV induced a probiotic effector gene and a likely deoxycholate transporter, two functions that improve the host health status. Conclusion Our data identify novel protective mechanisms involved in RSV tolerance operating at system level in a gut microbe. These insights could influence the way RSV is used for a better management of gut microbial ecosystems to obtain associated health benefits.