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The Oral Bioavailability of 8‐Prenylnaringenin from Hops ( Humulus Lupulus L.) in Healthy Women and Men is Significantly Higher than that of its Positional Isomer 6‐Prenylnaringenin in a Randomized Crossover Trial
Author(s) -
CalvoCastro Laura A.,
Burkard Markus,
Sus Nadine,
Scheubeck Gabriel,
Leischner Christian,
Lauer Ulrich M.,
BosyWestphal Anja,
Hund Verena,
Busch Christian,
Venturelli Sascha,
Frank Jan
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700838
Subject(s) - humulus lupulus , bioavailability , placebo , peripheral blood mononuclear cell , crossover study , xanthohumol , hop (telecommunications) , medicine , pharmacology , zoology , chemistry , gastroenterology , biochemistry , food science , biology , in vitro , pathology , computer network , alternative medicine , pepper , computer science
Scope Prenylated chalcones and flavonoids from hop ( Humulus lupulus L.), such as 6‐prenylnaringenin (6‐PN) and 8‐prenylnaringenin (8‐PN), are investigated for their health beneficial and anticancer activities. We, thus, compare the oral bioavailability and safety of 6‐PN and 8‐PN in healthy young women and men, and investigated their effects on peripheral blood mononuclear cells (PBMC). Methods and results A double‐blind, placebo‐controlled, crossover trial is conducted with 16 healthy volunteers (eight women, eight men) given a single oral dose of 500 mg 6‐PN, 8‐PN, or placebo in random order. Maximum total concentrations of 6‐PN and 8‐PN in plasma ( C max ; 543 and 2834 nmol L –1 ) and their respective area under the plasma concentration‐time curve (AUC; 3635 and 15801 nmol L –1 × h) are significantly (5.2‐ and 4.3‐fold) higher for 8‐PN than for 6‐PN ( p ˂ 0.05). PBMC for ex vivo experiments are isolated from blood sampled before and 6 h after intake of 6‐PN, 8‐PN, or placebo. Despite the single‐treatment regime and low blood concentrations, both 6‐PN and 8‐PN increase the survival of PBMC relative to control. Conclusion 8‐PN is significantly more bioavailable in healthy humans than its isomer 6‐PN. Interestingly, 6‐PN, despite being less bioavailable, is similarly effective as 8‐PN in enhancing PBMC viability.