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The Efficacy of Nanoemulsion‐Based Delivery to Improve Vitamin D Absorption: Comparison of In Vitro and In Vivo Studies
Author(s) -
Kadappan Alagu Selvi,
Guo Chi,
Gumus Cansu E.,
Bessey Amy,
Wood Richard J.,
McClements David J.,
Liu Zhenhua
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700836
Subject(s) - bioavailability , in vivo , vitamin d and neurology , absorption (acoustics) , emulsion , in vitro , vitamin , micelle , chemistry , pharmacology , chromatography , medicine , biochemistry , materials science , microbiology and biotechnology , biology , composite material , aqueous solution
Scope Vitamin D (VD) is a fat‐soluble vitamin that has a wide range of skeletal and non‐skeletal functions. Although it can be synthesized through sun exposure and obtained from fortified foods, VD inadequacy is epidemic worldwide. Therefore innovative strategies are necessary for improving VD status. The present study examined VD absorption via nanoscale delivery systems. Methods and results We examine the physical characteristics and in vitro bioaccessibility of cholecalciferol (VD 3 ) in nanoemulsion using a simulated gastrointestinal tract system. To evaluate the in vivo bioavailability, we orally administer three groups of mice with VD 3 nanoemulsion, VD 3 coarse emulsion, or vehicle nanoemulsion without VD 3 , and the serum 25(OH)D 3 is measured using radioactive immunoassay. The nanoemulsion‐based delivery system increases the in vitro bioaccessibility by 3.94‐folds ( p < 0.05), as indicated by the concentration of vitamin D 3 in micelles. Our animal study shows that, when compared to the vehicle group, the coarse emulsion numerically increases the serum 25(OH)D 3 by 36%, whereas the nanoemulsion statistically significantly increases the serum 25(OH)D 3 by 73% ( p < 0.01). Conclusion Our findings indicate that a nanoemulsion‐based delivery system is a promising approach to improve VD bioavailability, and further studies are warranted to determine its efficacy in humans.