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Bioavailability of Terpenes and Postprandial Effect on Human Antioxidant Potential. An Open‐Label Study in Healthy Subjects
Author(s) -
Papada Efstathia,
Gioxari Aristea,
Brieudes Vincent,
Amerikanou Charalampia,
Halabalaki Maria,
Skaltsounis Alexios Leandros,
Smyrnioudis Ilias,
Kaliora Andriana C.
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700751
Subject(s) - terpene , postprandial , bioavailability , ingestion , chemistry , antioxidant , phytochemical , oxidative stress , pharmacology , food science , medicine , biochemistry , insulin
Scope To assess bioavailability of terpenes in human plasma and their effect on oxidative stress biomarkers. Methods and results In this open‐label and single arm postprandial trial, seventeen healthy male volunteers (20–40 years old) follow a low‐phytochemical diet for 5 days. Next, after overnight fasting, volunteers consume Mastiha powder (a natural resin rich in terpenes) dispersed in water. Blood samples are collected on time points 0 h (before ingestion) and 0.5, 1, 2, 4, 6, and 24 h (post‐ingestion). Ultra‐high‐pressure liquid chromatography high‐resolution MS (UHPLC‐HRMS/MS) is applied for high throughput analysis of plasma. Serum resistance to oxidation and oxidized LDL (oxLDL) levels are measured. UHPLC‐HRMS/MS analysis shows that major terpenes are bioavailable since 0.5 h after administration, reaching a peak between 2 h and 4 h. Serum resistance to oxidation, expressed as difference of tLAG (time point‐0 h), starts to increase from 0.5 h. This increase reaches statistical significance at 4 h (402.3 ± 65.0 s), peaks at 6 h (524.6 ± 62.9 s), and remains statistically significant until 24 h (424.2 ± 48.0 s). oxLDL levels, expressed as %change from 0 h, are reduced significantly from time point‐1 h until time point‐6 h. Conclusion Results demonstrate the terpene bioavailability pattern after oral administration of Mastiha. Terpenes are potential mediators of antioxidant defense in vivo.