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Regulatory Effects of Black Rice Extract on Helicobacter pylori Infection‐Induced Apoptosis
Author(s) -
Kim SaHyun,
Lee Min Ho,
Park Min,
Woo Hyun Jun,
Kim Yoon Suk,
Tharmalingam Nagendran,
Seo WooDuck,
Kim JongBae
Publication year - 2018
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700586
Subject(s) - helicobacter pylori , apoptosis , flow cytometry , caga , survivin , xiap , blot , western blot , microbiology and biotechnology , biology , virulence , mtt assay , secretion , gene , chemistry , caspase , biochemistry , programmed cell death , genetics
Scope Black rice extract (BRE) contains cyanidin 3‐ O ‐glucoside (C3G), an anthocyanin, as the major component. In this study, we found that BRE inhibits the mRNA and protein expression of genes encoding cytotoxin‐associated protein A ( cagA ) and vacuolating protein A ( vacA ) in Helicobacter pylori 60190 strain. Methods and results We performed RT‐PCR and western blotting to show that BRE inhibits the mRNA and protein expression of SecA. Because SecA is involved in VacA export in bacteria, our result suggests a positive correlation between BRE‐induced inhibition of secA expression and VacA secretion. Further, we perform MTT assay and flow cytometry to show that BRE decreases the apoptosis of H. pylori –infected KATO III cells. Finally, we perform western blotting to show that the cell‐protective effect of BRE is associated with decreased levels of active proapoptotic proteins caspases and PARP and increased levels of antiapoptotic proteins survivin and XIAP in H. pylori –infected cells. Conclusion Thus, our results indicate that BRE acts as a potent inhibitor of the biogenesis of H. pylori virulence proteins and decreases the apoptosis of H. pylori –infected cells. Moreover, our results suggest that BRE can be used to exert beneficial effects in patients with gastroduodenal diseases caused by H. pylori .

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