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Chain length‐dependent effects of inulin‐type fructan dietary fiber on human systemic immune responses against hepatitis‐B
Author(s) -
Vogt Leonie M.,
Elderman Marlies E.,
Borghuis Theo,
Haan Bart J.,
Faas Marijke M.,
Vos Paul
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700171
Subject(s) - immune system , immunity , immunology , hbsag , inulin , medicine , biology , hepatitis b virus , food science , virus
Scope In vivo studies demonstrating that only specific dietary‐fibers contribute to immunity are still inconclusive, as measuring immune effects in healthy humans remains difficult. We applied a relatively inefficacious vaccination‐challenge to study chain length‐dependent effects of inulin‐type fructan (ITF) dietary fibers on human immunity. Methods and results ITFs with two different ‘degree of polymerization‐’ (DP)‐profiles were tested in vitro for effects on PBMC‐cytokines and TLR2 activation. In a double‐blind placebo‐controlled trial, 40 healthy volunteers (18–29 years) were divided into three groups and supplemented from day 1 to day 14 with DP10‐60 ITF, DP2‐25 ITF (both n = 13), or fructose placebo (n = 14), 8 g/day. On day 7, all volunteers were vaccinated against hepatitis B. Anti‐HbsAg‐titer development and lymphocyte subsets were studied. In vitro, DP10‐60 ITFs stimulated a Th1‐like cytokine profile and stimulated TLR2 more strongly than DP2‐25 ITFs. In vivo, DP10‐60 increased anti‐HBsAg titers, Th1‐cells, and transitional B‐cells. Both ITFs increased CD45RO hi CTLs at day 35, and CD161 + cytokine producing NK‐cells at day 21 and 35. Conclusion Support of immunity is determined by the chain length of ITFs. Only long‐chain ITFs support immunity against pathogenic hepB‐epitopes introduced by vaccination. Our findings demonstrate that specific dietary fibers need to be selected for immunity support.