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Dietary advanced glycation end products modify gut microbial composition and partially increase colon permeability in rats
Author(s) -
Qu Wanting,
Yuan Xiaojin,
Zhao Jinsong,
Zhang Yingxiao,
Hu Jing,
Wang Jiawei,
Li Juxiu
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700118
Subject(s) - gut flora , food science , bacteria , bacteroides , glycation , biology , fermentation , lactobacillus , microbiology and biotechnology , chemistry , biochemistry , genetics , receptor
Scope The adverse impacts of dietary advanced glycation end products (AGEs) on health are currently of interest. These compounds are inevitably formed during thermal food processing and make foods less digestible because of protein cross‐linking. This study examined not only whether dietary AGEs alter cecal microbiota and their metabolites but also their effects on colon permeability. Methods and results Sprague–Dawley rats were exposed to a high‐AGEs diet (AGEs content was increased by heating food at 125°C/3 h) for 6, 12, or 18 weeks. Cecal microbiota was analyzed by 16S rDNA gene sequencing. Colon permeability was assessed through histopathology, immunohistochemistry and endotoxin testing. Microbial metabolites (e.g. ammonia and short‐chain fatty acids (SCFAs)) were also measured. AGEs treatment reduced the diversity and richness of the microbiota, especially saccharolytic bacteria such as Ruminococcaceae and Alloprevotella , which can produce SCFAs, whereas some putatively harmful bacteria ( Desulfovibrio and Bacteroides ) were increased. Protein fermentation was enhanced, supported by elevated ammonia and branched‐chain fatty acid levels ( p < 0.05). Additionally, the colonocytes structure changed and the expression of tight junction proteins in colon were decreased. Conclusion Dietary AGEs detrimentally modulate gut microbial ecology and may partially increase colon permeability, which can adversely impact host health.