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Pru p 3‐Epitope‐based sublingual immunotherapy in a murine model for the treatment of peach allergy
Author(s) -
Rodriguez Maria J.,
Mascaraque Ainhoa,
RamosSoriano Javier,
Torres Maria J.,
Perkins James R.,
Gomez Francisca,
GarridoArandia Maria,
CubellsBaeza Nuria,
Andreu David,
DiazPerales Araceli,
Rojo Javier,
Mayorga Cristobalina
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201700110
Subject(s) - immunoglobulin e , epitope , immunotherapy , immunology , allergy , food allergy , cpg oligodeoxynucleotide , anaphylaxis , antibody , medicine , t cell , cpg site , peptide , pharmacology , chemistry , immune system , biochemistry , gene , gene expression , dna methylation
Scope Food‐specific immunotherapy (SIT) is a promising treatment for lipid transfer protein (LTP)‐syndrome. We propose a novel sublingual‐SIT (SLIT) that combines a Pru p 3 T‐cell peptide and an oligodeoxyribonucleotide (ODN) with CpG motifs (ODN‐CpG) as adjuvants to induce a specific Th1/Treg response. Methods and results LTP‐peach allergic mice were treated sublingually with a combination of a CpG sequence and mono‐ or tetravalent systems including a Pru p 3 peptide, D 1 (Prup3) or D 4 (Prup3). Mice were challenged intraperitoneally with Pru p 3 one or three weeks after SLIT and tolerance was assessed. Mice treated with D 1 (Prup3)+CpG were protected from anaphylaxis after Pru p 3 challenge. They showed no change in body temperature, lower levels of Pru p 3‐specific IgE and IgG1 antibodies and higher levels of sIgG2a compared to the untreated group. They had fewer IgE and IgG1 secreting cells and more sIgG2a secreting cells. Moreover, a significantly lower number of Pru p 3‐specific CD4 + T cells and a higher number of Treg cells were found, alongside a Th1 cytokine pattern. These changes were maintained for three weeks after stopping treatment. Conclusion D 1 Prup3+CpG represents a promising SIT for food allergy. It is easily synthesized and induces protection from anaphylaxis to Pru p 3 that is maintained for at least three weeks.