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Alliin, a garlic organosulfur compound, ameliorates gut inflammation through MAPK‐NF‐κB/AP‐1/STAT‐1 inactivation and PPAR‐γ activation
Author(s) -
Shi Limin,
Lin Qinlu,
Li Xinhua,
Nie Ying,
Sun Shuguo,
Deng Xiyun,
Wang Long,
Lu Jun,
Tang Yiping,
Luo Feijun
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201601013
Subject(s) - alliin , chemistry , p38 mitogen activated protein kinases , mapk/erk pathway , colitis , nf κb , pharmacology , lipopolysaccharide , inflammation , iκbα , blot , kinase , biochemistry , microbiology and biotechnology , signal transduction , immunology , allicin , biology , gene
Scope In this study, the anti‐inflammatory effects and the molecular mechanism of alliin were analyzed in dextran sulfate sodium (DSS)‐induced colitis mice and lipopolysaccharide‐stimulated RAW264.7 cell model. Methods The phenotype of mice was recorded in the DSS‐induced and/or alliin (500 mg/kg) groups. Histopathological alterations were analyzed by H&E staining. MPO and MDA of colon tissues were measured. The mRNA expression levels of inflammatory factors were determined by qRT‐PCR, and protein expressions of inflammatory factors or activation of kinases were determined by Western blotting. Results Oral administration of alliin significantly inhibited the decrease of body weight, improved the DAI and decreased the infiltration of inflammatory cells in colonic tissues. The content of NO, MDA, and MPO, the expression of iNOS and inflammatory factors as well as MAPK and the phosphorylation of PPAR‐γ were inhibited in alliin‐treated group. Treatment with alliin significantly repressed the expression of inflammatory factors in LPS‐stimulated RAW264.7 cells. Further research demonstrated that alliin repressed LPS‐induced AP‐1/NF‐κB/STAT‐1 activation by inhibiting the phosphorylations of p38, JNK, and ERK1/2‐regulated PPAR‐γ activation. Conclusion Our results show that alliin ameliorates DSS‐induced ulcerative colitis and inhibits the inflammatory responses in LPS‐stimulated RAW264.7 cells partly through inhibiting ERK1/2‐, JNK‐/PPAR‐γ–stimulated NF‐κB/AP‐1/STAT‐1 activations.