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Distribution of trans ‐resveratrol and its metabolites after acute or sustained administration in mouse heart, brain, and liver
Author(s) -
Menet MarieClaude,
Baron Stephanie,
Taghi Meryam,
Diestra Remi,
Dargère Delphine,
Laprévote Olivier,
NivetAntoine Valérie,
Beaudeux JeanLouis,
Bédarida Tatiana,
Cottart CharlesHenry
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600686
Subject(s) - resveratrol , pharmacology , chemistry , distribution (mathematics) , metabolism , metabolite , biochemistry , medicine , mathematical analysis , mathematics
Scope Trans ‐resveratrol is widely studied for its potentially beneficial effects on numerous disorders. It is rapidly metabolized and its metabolites can exhibit biological activity. The present study aimed to investigate whether acute or sustained trans ‐resveratrol administration impacted on the distribution of trans ‐resveratrol and its metabolites in brain, heart, and liver. Methods and results We used ultra‐HPLC quadrupole‐TOF (UHPLC‐Q‐TOF) in a full‐scan mode to identify and assess large numbers of resveratrol metabolites. For acute intake, mice were overfed with a single dose of trans ‐resveratrol (150 mg/kg) and organs were collected after 30 and 60 min. For sustained intake, trans ‐resveratrol was given in the chow (0.04% w/w corresponding to 40 mg/kg/day), and plasma and the organs were collected after 3 months of this resveratrol diet. We found that trans ‐resveratrol‐3‐ O ‐glucuronide and resveratrol‐3‐sulfate were the main metabolites found after acute intake, and free trans ‐resveratrol (in the brain and heart) and dihydroresveratrol derivatives were found after sustained administration Conclusions Our results show notable differences between acute and sustained administration of trans ‐resveratrol and distribution of trans ‐resveratrol and its metabolites in mouse heart, brain, and liver. The results suggest a strategy for development of galenic forms of resveratrol.