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Human peripheral blood mononuclear cell in vitro system to test the efficacy of food bioactive compounds: Effects of polyunsaturated fatty acids and their relation with BMI
Author(s) -
Cifre Margalida,
DíazRúa Rubén,
VarelaCalviño Rubén,
Reynés Bàrbara,
PericásBeltrán Jordi,
Palou Andreu,
Oliver Paula
Publication year - 2017
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600353
Subject(s) - peripheral blood mononuclear cell , docosahexaenoic acid , polyunsaturated fatty acid , eicosapentaenoic acid , endocrinology , medicine , inflammation , population , lipid metabolism , fatty acid , biology , immunology , chemistry , in vitro , biochemistry , environmental health
Scope To analyse the usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n –3 long‐chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are body mass index (BMI) dependent. Methods and results PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination. PBMC reflected increased beta‐oxidation‐like capacity ( CPT1A expression) in OW/OB but only after DHA treatment. However, insensitivity to n –3 LCPUFA was evident in OW/OB for lipogenic genes: both PUFA diminished FASN and SREBP1C expression in NW, but no effect was observed for DHA in PBMC from high‐BMI subjects. This insensitivity was also evident for inflammation gene profile: all treatments inhibited key inflammatory genes in NW; nevertheless, no effect was observed in OW/OB after DHA treatment, and EPA effect was impaired. SLC27A2 , IL6 and TNFα PBMC expression analysis resulted especially interesting to determine obesity‐related n –3 LCPUFA insensitivity. Conclusion A PBMC‐based human in vitro system reflects n –3 LCPUFA effects on lipid metabolism/inflammation which is impaired in OW/OB. These results confirm the utility of PBMC ex vivo systems for bioactive‐compound screening to promote functional food development and to establish appropriate dietary strategies for obese population.