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Coffee consumption modulates inflammatory processes in an individual fashion
Author(s) -
Muqaku Besnik,
Tahir Ammar,
Klepeisz Philip,
Bileck Andrea,
Kreutz Dominique,
Mayer Rupert L.,
Meier Samuel M.,
Gerner Marlene,
Schmetterer Klaus,
Gerner Christopher
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600328
Subject(s) - caffeine , inflammation , chemokine , peripheral blood mononuclear cell , ex vivo , stimulation , immunology , downregulation and upregulation , prostaglandin e2 , pharmacology , in vitro , medicine , biology , biochemistry , gene
Scope Anti‐inflammatory effects of coffee consumption have been reported to be caused by caffeine and adenosine receptor signaling. However, contradictory effects have been observed. Many kinds of chronic diseases are linked to inflammation; therefore a profound understanding of potential effects of coffee consumption is desirable. Methods and results We performed ex vivo experiments with eight individuals investigating peripheral blood mononuclear cells isolated from venous blood before and after coffee consumption, as well as in vitro experiments applying caffeine on isolated cells. After in vitro inflammatory stimulation of the cells, released cytokines, chemokines, and eicosanoids were determined and quantified using targeted mass spectrometric methods. Remarkably, the release of inflammation mediators IL6, IL8, GROA, CXCL2, CXCL5 as well as PGA2, PGD2, prostaglandin E2 (PGE2), LTC4, LTE4, and 15S‐HETE was significantly affected after coffee consumption. While in several individuals coffee consumption or caffeine treatment caused significant downregulation of most inflammation mediators, in other healthy individuals exactly the opposite effects were observed. Conclusion Ruling out age, sex, coffee consumption habits, the metabolic kinetics of caffeine in blood and the individual amount of regulatory T cells or CD39 expression as predictive parameters, we demonstrated here that coffee consumption may have significant pro‐ or anti‐inflammatory effects in an individual fashion.