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Acute effects of dietary fatty acids on osteclastogenesis via RANKL/RANK/OPG system
Author(s) -
Naranjo M. Carmen,
Garcia Indara,
Bermudez Beatriz,
Lopez Sergio,
Cardelo Magdalena P.,
Abia Rocio,
Muriana Francisco J. G.,
Montserratde la Paz Sergio
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600303
Subject(s) - postprandial , rankl , osteoclast , endocrinology , medicine , polyunsaturated fatty acid , osteoprotegerin , chemistry , rank ligand , fatty acid , biochemistry , biology , receptor , activator (genetics) , insulin
Scope Postprandial state is directly linked with chronic diseases. We hypothesized that dietary fats may have acute effects on health status by modulating osteoclast differentiation and activation in a fatty acid‐dependent manner. Methods and results In healthy subjects, a fat‐enriched meal increased plasma levels of the RANKL (receptor activator of nuclear factor κB ligand)/OPG (osteoprotegerin) ratio (SFAs > MUFAs = PUFAs) in the postprandial state. Postprandial TRL‐SFAs enhanced tartrate‐resistant acid phosphatase (TRAP) activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte‐derived osteoclasts. These effects were not observed with monounsaturated fatty acid (MUFA)‐enriched postprandial triglyceride‐rich lipoproteins (TRLs). Moreover, postprandial TRL‐SFAs increased the release of osteoclastogenic cytokines (TNF‐α, IL‐1β, and IL‐6) meanwhile TRL‐MUFAs and TRL‐PUFAs increased the release of anti‐osteoclastogenic cytokines (IL‐4 and IL‐10) in the medium of human monocyte‐derived osteoclasts. Conclusion For the first time, we show that postprandial TRLs are metabolic entities with osteoclastogenic activity and that this property is related to the type of dietary fatty acid in the meal. The osteoclastogenic potency was as follows: SFAs >>> MUFAs = PUFAs. These exciting findings open opportunities for developing nutritional strategies with olive oil as the principal dietary source of MUFAs, notably oleic acid, to prevent development and progression of osteoclast‐related diseases.