Premium
High phenolics Rutgers Scarlet Lettuce improves glucose metabolism in high fat diet‐induced obese mice
Author(s) -
Cheng Diana M.,
Roopchand Diana E.,
Poulev Alexander,
Kuhn Peter,
Armas Isabel,
Johnson William D.,
Oren Andrew,
Ribnicky David,
Zelzion Ehud,
Bhattacharya Debashish,
Raskin Ilya
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600290
Subject(s) - food science , digestion (alchemy) , gut flora , biology , polysaccharide , gastrointestinal tract , carbohydrate metabolism , dysbiosis , chlorogenic acid , chemistry , biochemistry , chromatography
Scope The ability of high phenolic Rutgers Scarlet Lettuce (RSL) to attenuate metabolic syndrome and gut dysbiosis was studied in very high fat diet (VHFD)‐fed mice. Phenolic absorption was assessed in vivo and in a gastrointestinal tract model. Methods and results Mice were fed VHFD, VHFD supplemented with RSL (RSL‐VHFD) or store‐purchased green lettuce (GL‐VHFD), or low‐fat diet (LFD) for 13 weeks. Compared to VHFD or GL‐VHFD‐fed groups, RSL‐VHFD group showed significantly improved oral glucose tolerance ( p <0.05). Comparison of VHFD, RSL‐VHFD, and GL‐VHFD groups revealed no significant differences with respect to insulin tolerance, hepatic lipids, body weight gain, fat mass, plasma glucose, triglycerides, free fatty acid, and lipopolysaccharide levels, as well as relative abundances of major bacterial phyla from 16S rDNA amplicon data sequences (from fecal and cecal samples). However, RSL and GL‐supplementation increased abundance of several taxa involved in plant polysaccharide degradation/fermentation. RSL phenolics chlorogenic acid, quercetin‐3‐glucoside, and quercetin‐malonyl‐glucoside were bioaccessible in the TIM‐1 digestion model, but had relatively low recovery. Conclusions RSL phenolics contributed to attenuation of post‐prandial hyperglycemia. Changes in gut microbiota were likely due to microbiota accessible carbohydrates in RSL and GL rather than RSL phenolics, which may be metabolized, absorbed, or degraded before reaching the colon.