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Different effects of anthocyanins and phenolic acids from wild blueberry ( Vaccinium angustifolium ) on monocytes adhesion to endothelial cells in a TNF‐α stimulated proinflammatory environment
Author(s) -
Del Bo' Cristian,
Roursgaard Martin,
Porrini Marisa,
Loft Steffen,
Møller Peter,
Riso Patrizia
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600178
Subject(s) - syringic acid , gallic acid , proinflammatory cytokine , malvidin , umbilical vein , adhesion , chemistry , tumor necrosis factor alpha , biochemistry , protocatechuic acid , cell adhesion , cyanidin , biology , inflammation , immunology , organic chemistry , antioxidant , in vitro
Scope Monocyte adhesion to the vascular endothelium is a crucial step in the early stages of atherogenesis. This study aims to investigate the capacity of an anthocyanin (ACN) and phenolic acid (PA) rich fraction (RF) of a wild blueberry, single ACNs (cyanidin, malvidin, delphinidin) and related metabolites (protocatechuic, syringic, and gallic acid) to counteract monocytes (THP‐1) adhesion to human umbilical vein endothelial cells (HUVECs) in a tumor necrosis factor α (TNF‐α) mediated proinflammatory environment. Methods and results HUVECs were incubated with different concentrations (from 0.01 to 10 μg/mL) of the compounds for 24 h. Labeled monocytic THP‐1 cells were added to HUVECs and their adhesion was induced by TNF‐α (100 ng/mL). ACN‐RF reduced THP‐1 adhesion to HUVECs with a maximum effect at 10 μg/mL (−33%). PA‐RF counteracted THP‐1 adhesion at 0.01, 0.1, and 1 μg/mL (−45, −48.7, and −27.6%, respectively), but not at maximum concentration. Supplementation with gallic acid reduced THP‐1 adhesion to HUVECs with a maximum effect at 1 μg/mL (−29.9%), while malvidin‐3‐glucoside and syringic acid increased the adhesion. No effect was observed for the other compounds. Conclusion These results suggest that ACNs/PA‐RF may prevent atherogenesis while the effects of the single ACNs and metabolites are controversial and merit further exploration.