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Quantitative assessment of organ distribution of dietary protein‐bound 13 C‐labeled N ɛ ‐carboxymethyllysine after a chronic oral exposure in mice
Author(s) -
Tessier Frédéric J.,
NiquetLéridon Céline,
Jacolot Philippe,
Jouquand Céline,
Genin Michaël,
Schmidt AnnMarie,
Grossin Nicolas,
Boulanger Eric
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201600140
Subject(s) - lysine , glycation , chemistry , kidney , urine , biochemistry , medicine , amino acid , endocrinology , biology , receptor
Scope N ɛ ‐Carboxymethyl‐lysine (CML) is a prominent advanced glycation end‐product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found. Methods and results A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass‐to‐charge ratios were used: nCML N ε ‐carboxymethyl‐L‐lysine, dCML N ε ‐[ 13 C]carboxy[ 13 C]methyl‐L‐lysine and internal standard N ε ‐carboxymethyl‐L‐[4,4,5,5‐ 2 H 4 ]lysine. Wild‐type ( n = 7) and RAGE −/− ( n = 8) mice were fed for 30 days with either a control, or a BSA‐bound dCML‐enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography–tandem mass spectrometry. Mice exposed to dCML showed an accumulation in all tissues tested except fat. The rate of deposition was high (81–320 μg dCML /g dry matter) in kidneys, intestine, and lungs and low (<5 μg/g) in heart, muscle, and liver. This accumulation was not RAGE dependent. Conclusion The kidney is not the only organ affected by the accumulation of dCML. Its high accumulation in other tissues and organs may also, however, have important physiological consequences.