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Lean‐seafood intake decreases urinary markers of mitochondrial lipid and energy metabolism in healthy subjects: Metabolomics results from a randomized crossover intervention study
Author(s) -
Schmedes Mette,
Aadland Eli Kristin,
Sundekilde Ulrik Kræmer,
Jacques Hélène,
Lavigne Charles,
Graff Ingvild Eide,
Eng Øyvin,
Holthe Asle,
Mellgren Gunnar,
Young Jette Feveile,
Bertram Hanne Christine,
Liaset Bjørn,
Clausen Morten Rahr
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500785
Subject(s) - urinary system , lipid metabolism , crossover study , urine , carnitine , metabolomics , catabolism , excretion , food science , medicine , endocrinology , trimethylamine n oxide , chemistry , metabolism , lipid oxidation , trimethylamine , biology , biochemistry , bioinformatics , antioxidant , placebo , alternative medicine , pathology
Scope Proteins constitute an important part of the human diet, but understanding of the effects of different dietary protein sources on human metabolism is sparse. We aimed to elucidate diet‐induced metabolic changes through untargeted urinary metabolomics after four weeks of intervention with lean‐seafood or nonseafood diets. It is shown that lean‐seafood intake reduces urinary excretion of metabolites involved in mitochondrial lipid and energy metabolism possibly facilitating a higher lipid catabolism in healthy subjects. Methods In a randomized controlled trial with crossover design, 20 healthy subjects consumed two balanced diets that varied in main protein sources for 4 weeks. Morning spot urine samples were collected before and after each intervention period. Untargeted metabolomics based on 1 H NMR spectroscopy and LC‐MS analyses were applied to characterize the urinary metabolic response to the interventions. Results The lean‐seafood diet period reduced the urinary level of l ‐carnitine, 2,6‐dimethylheptanoylcarnitine, and N ‐methyl‐2‐pyridone‐5‐carboxamide, relative to the nonseafood period. The dietary analysis revealed that the higher urinary level of trimethylamine‐ N ‐oxide after the lean‐seafood diet period and guanidinoacetate and 3‐methylhistidine after the nonseafood diet period was related to the endogenous content of these compounds in the diets. Conclusions Our data reveal that 4 weeks of lean‐seafood intake reduces urinary excretion of metabolites involved in mitochondrial lipid and energy metabolism possibly facilitating a higher lipid catabolism in healthy subjects after the lean‐seafood intake.