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Protective effects of β‐casofensin, a bioactive peptide from bovine β‐casein, against indomethacin‐induced intestinal lesions in rats
Author(s) -
Bessette Claudine,
Benoit Bérengère,
Sekkal Soraya,
Bruno Jérémie,
Estienne Monique,
Léonil Joelle,
Ferrier Laurent,
Théodorou Vassilia,
Plaisancié Pascale
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500680
Subject(s) - jejunum , pharmacology , ileum , indometacin , cytoprotection , goblet cell , chemistry , medicine , endocrinology , in vitro , pathology , epithelium , oxidative stress , biochemistry , prostaglandin endoperoxide synthase , enzyme inhibitor
Scope β‐casofensin, also known as peptide β‐CN(94‐123), is a milk bioactive peptide that modulates the intestinal barrier through its action on goblet cells. Here, we evaluated whether oral administration of β‐casofensin can prevent indomethacin‐induced injury of the jejunum in rats. Methods and results Rats received β‐casofensin (0.01–100 μM) or tap water by daily gavage (4 μL/g) for eight days, then two subcutaneous injections of indomethacin (10 mg/kg, days 9 and 10) and were euthanized on day 12. In vitro, we investigated the effects of β‐casofensin on the restitution of a wounded monolayer. Preventive administration of β‐casofensin (100 μM) reduced intestinal macroscopic and microscopic damage induced by indomethacin. β‐casofensin also prevented the depletion of goblet cells and increased myeloperoxidase activity, as well as tumor necrosis factor‐ɑ (TNF‐ɑ) expression and immunostaining of active caspase‐3 in the jejunum of rats treated with indomethacin. In wound healing experiments, β‐casofensin promoted epithelial restitution with no effect on cell proliferation. This effect was inhibited by pre‐incubation with an anti‐CC chemokine receptor 6 (CCR6) neutralizing antibody. Conclusions β‐casofensin exerts protective effects in indomethacin‐induced enteritis through preservation of goblet cells and improvement in wound healing. β‐casofensin could therefore become vital in nutritional programs for the prevention of intestinal diseases.