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Coffee consumption rapidly reduces background DNA strand breaks in healthy humans: Results of a short‐term repeated uptake intervention study
Author(s) -
Bakuradze Tamara,
Lang Roman,
Hofmann Thomas,
Schipp Dorothea,
Galan Jens,
Eisenbrand Gerhard,
Richling Elke
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500668
Subject(s) - comet assay , dna damage , medicine , zoology , toxicology , physiology , dna , food science , biology , biochemistry
Scope Intervention studies provide evidence that long‐term coffee consumption correlates with reduced DNA background damage in healthy volunteers. Here, we report on short‐term kinetics of this effect, showing a rapid onset after normal coffee intake. Methods and results In a short‐term human intervention study, we determined the effects of coffee intake on DNA integrity during 8 h. Healthy male subjects ingested coffee in 200 mL aliquots every second hour up to a total volume of 800 mL. Blood samples were taken at baseline, immediately before the first coffee intake and subsequently every 2 h, prior to the respective coffee intake. DNA integrity was assayed by the comet assay. The results show a significant ( p < 0.05) reduction of background DNA strand breaks already 2 h after the first coffee intake. Continued coffee intake was associated with further decrements in background DNA damage within the 8 h intervention ( p < 0.01 and p < 0.001, respectively). Mean tail intensities (TIs%) decreased from 0.33 TI% (baseline, 0 h) to 0.22 TI% (within 8 h coffee consumption). Conclusion Repeated coffee consumption was associated with reduced background DNA strand breakage, clearly measurable as early as 2 h after first intake resulting in a cumulative overall reduction by about one‐third of the baseline value.