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The chemopreventive activity of butyrate‐containing structured lipids in experimental rat hepatocarcinogenesis
Author(s) -
Heidor Renato,
Conti Aline,
Ortega Juliana F.,
Furtado Kelly S.,
Silva Roberta C.,
Tavares Paulo E. L. M.,
Purgatto Eduardo,
Ract Julia. R.,
Paiva Sérgio A. R.,
Gioielli Luiz A.,
Pogribny Igor P.,
Moreno Fernando S.
Publication year - 2016
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500643
Subject(s) - butyrate , chemistry , cancer research , computational biology , microbiology and biotechnology , biochemistry , biology , fermentation
Scope Emerging evidence indicates that the use of bioactive food components is a promising strategy to prevent the development of liver cancer. The goal of this study was to examine the chemopreventive effect of butyrate‐containing structured lipids (STLs) produced by an enzymatic interesterification of tributyrin and flaxseed oil on rat hepatocarcinogenesis. Methods and results Male Wistar rats were subjected to a classic “resistant hepatocyte” model of liver carcinogenesis and treated with STLs, tributyrin or flaxseed oil during the initial phases of hepatocarcinogenesis. Treatment with STLs and tributyrin strongly inhibited the development of preneoplastic liver lesions. The chemopreventive activity of tributyrin was associated with the induction of apoptosis and reduction of the expression of major activated hepatocarcinogenesis‐related oncogenes. Treatment with STLs caused substantially greater inhibitory effects than tributyrin on oncogene expression. Conclusion These results demonstrate that the tumor‐suppressing activity of butyrate‐containing STLs is associated with its ability to prevent and inhibit activation of major hepatocarcinogenesis‐related oncogenes. Enrichment of histone H3K9me3 and H3K27me3 at the promoter of Myc and Ccnd1 genes may be related to the inhibitory effect on oncogene expression in the livers of STL‐treated rats.