Premium
Sex differences in skin carotenoid deposition and acute UVB‐induced skin damage in SKH‐1 hairless mice after consumption of tangerine tomatoes
Author(s) -
Kopec Rachel E.,
Schick Jonathan,
Tober Kathleen L.,
Riedl Ken M.,
Francis David M.,
Young Gregory S.,
Schwartz Steven J.,
Oberyszyn Tatiana M.
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500317
Subject(s) - hairless , carotenoid , deposition (geology) , chemistry , consumption (sociology) , food science , dermatology , biology , medicine , biochemistry , art , paleontology , sediment , aesthetics
Scope UVB exposure, a major factor in the development of skin cancer, has differential sex effects. Tomato product consumption reduces the intensity of UVB‐induced erythema in humans, but the mechanisms are unknown. Methods and results Four‐week‐old SKH‐1 hairless mice (40 females, 40 males) were divided into two feeding groups (control or with 10% tangerine tomatoes naturally rich in UV‐absorbing phytoene and phytofluene) and two UV exposure groups (with or without UV). After 10 weeks of feeding, the UV group was exposed to a single UV dose and sacrificed 48 h later. Blood and dorsal skin samples were taken for carotenoid analysis. Dorsal skin was harvested to assess sex and UV effects on carotenoid deposition, inflammation (skinfold thickness, myeloperoxidase levels), and DNA damage (cyclobutane pyrimidine dimers, p53). Females had significantly higher levels of both skin and blood carotenoids relative to males. UV exposure significantly reduced skin carotenoid levels in females but not males. Tomato consumption attenuated acute UV‐induced increases in CPD in both sexes, and reduced myeloperoxidase activity and percent p53 positive epidermal cells in males. Conclusion Tangerine tomatoes mediate acute UV‐induced skin damage in SKH‐1 mice via reduced DNA damage in both sexes, and through reduced inflammation in males.