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Preventive effects of dietary hydroxytyrosol acetate, an extra virgin olive oil polyphenol in murine collagen‐induced arthritis
Author(s) -
Rosillo María Angeles,
SánchezHidalgo Marina,
GonzálezBenjumea Alejandro,
FernándezBolaños José G.,
Lubberts Erik,
AlarcóndelaLastra Catalina
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500304
Subject(s) - hydroxytyrosol , janus kinase , endocrinology , medicine , stat protein , kinase , heme oxygenase , chemistry , proinflammatory cytokine , immunology , signal transduction , cytokine , inflammation , antioxidant , stat3 , biochemistry , heme , polyphenol , enzyme
Scope Hydroxytyrosol acetate (HTy‐Ac), an extra virgin olive oil (EVOO) polyphenol, has recently been reported to exhibit antioxidant and anti‐inflammatory effects on LPS‐stimulated macrophagesand ulcerative colitis. This study was designed to evaluate dietary HTy‐Ac supplementation effects on collagen‐induced arthritis (CIA) in mice. Methods and results DBA‐1/J mice were fed from weaning with 0.05% HTy‐Ac. After 6 weeks, arthritis was induced by type II collagen. Mice were sacrificed 42 days after first immunization. Blood was recollected and paws were histological and biochemically processed. HTy‐Ac diet significantly prevent edarthritis development and decreased serum IgG1 and IgG2a, cartilage olimeric matrix protein (COMP) and metalloproteinase‐3 (MMP‐3) levels, as well as, pro‐inflammatory cytokines levels (TNF‐α, IFN‐γ, IL‐1β, IL‐6 and IL‐17A). The activation of Janus kinase‐signal transducer and activator of transcription (JAK/STAT), mitogen‐activated protein kinases (MAPKs) and nuclear transcription factor‐kappa B (NF‐κB) pathways were drastically ameliorated whereas nuclear factor E2‐related factor 2 (Nrf2) and heme oxygenase‐1 (HO‐1) protein expressions were significantly up‐regulated in those mice fed with HTy‐Ac. Conclusion HTy‐Ac improved the oxidative events and returned pro‐inflammatory proteins expression to basal levels probably through JAK/STAT, MAPKs and NF‐κB pathways. HTy‐Ac supplement might provide a basis for developing a new dietary strategy for the prevention of rheumatoid arthritis.