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Deoxynivalenol inhibits the expression by goblet cells of intestinal mucins through a PKR and MAP kinase dependent repression of the resistin‐like molecule β
Author(s) -
Pinton Philippe,
Graziani Fabien,
Pujol Ange,
Nicoletti Cendrine,
Paris Océane,
Ernouf Pauline,
Di Pasquale Eric,
Perrier Josette,
Oswald Isabelle P.,
Maresca Marc
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201500005
Subject(s) - mucin , goblet cell , muc1 , biology , protein kinase a , microbiology and biotechnology , kinase , protein kinase r , barrier function , mucus , p38 mitogen activated protein kinases , gene silencing , biochemistry , gene , cyclin dependent kinase 2 , epithelium , genetics , ecology
Scope The food‐associated mycotoxin deoxynivalenol (DON) is known to affect intestinal functions. However, its effect on intestinal mucus is poorly characterized. Methods and results We analyzed the effects of DON on human goblet cells (HT29‐16E cells) and porcine intestinal explants. Results showed that subtoxic doses of DON (as low as 1 μM) decreased mucin (MUC) production. qPCR analysis demonstrated that this inhibition was due to a specific decrease in the level of mRNA encoding for the intestinal membrane‐associated (MUC1) and the secreted MUCs (MUC2, MUC3). Mechanistic studies demonstrated that DON effect relied on the activation of the protein kinase R and the mitogen‐activated protein kinase p38 ultimately leading to the inhibition of the expression of resistin‐like molecule beta, a known positive regulator of MUC expression. Conclusion Taken together, our results show that at low doses found in food and feed, DON is able to affect the expression and production of MUCs by human and animal goblet cells. Due to the important role of MUCs in the barrier function and in the interaction of commensal bacteria with the host, such effect could explain the observed modifications in the microbial diversity and the increased susceptibility to enteric infection following exposure to DON.