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Ascorbic acid modulates cell migration in differentiated HL‐60 cells and peripheral blood leukocytes
Author(s) -
Schwager Joseph,
Bompard Albine,
Weber Peter,
Raederstorff Daniel
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400893
Subject(s) - chemokinesis , chemotaxis , ascorbic acid , retinoic acid , chemistry , monocyte , microbiology and biotechnology , immunology , receptor , biology , biochemistry , food science , gene
Scope The impact of L‐ascorbic acid (L‐AA) on the chemokinesis (CK) and chemotaxis (CT) of HL‐60 cells and polymorphonuclear cells (PMN) was investigated. Methods and results HL‐60 cells were differentiated with DMSO, retinoic acid (RA), vitamin D, or L‐AA. Chemokinesis and chemotaxis of differentiated HL‐cells were assayed. Vitamin D3‐treated HL‐60 cells (dHL‐60 vitD3 cells) and RA‐treated cells (dHL‐60 RA cells) acquired monocyte/macrophage‐like and neutrophil‐like phenotypes, respectively. DMSO induced the differentiation of an intermediate phenotype (dHL‐60 DMSO cells), whereas L‐AA downregulated neutrophil markers (dHL‐60 L‐AA cells). dHL‐60 DMSO cells had increased CK and potent CT in gradients of IL‐8 and N‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine (fMLP). dHL‐60 RA cells and dHL‐60 L‐AA cells migrated less toward IL‐8 and fMLP; dHL‐60 vitD3 cells preferably responded to fMLP. L‐AA enhanced CK of dHL‐60 DMSO cells and was a weak chemo‐attractant. In human leukocytes, IL‐8 and fMLP triggered receptor‐mediated chemotaxis. CXCR2 and fMLPR were downregulated by IL‐8 and fMLP, respectively. L‐AA stimulated chemotaxis although significantly less than IL‐8 and fMLP. IL‐8 targeted chemotaxis was enhanced both in HL‐60 cells and leukocytes when cells were incubated with L‐AA. Conclusion L‐AA modulated chemokinesis and had significant chemo‐attractant properties, which were independent on fMLP or IL‐8 receptors. The results suggest that L‐AA improves leukocyte function in innate immune responses.