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Role of intestinal brush border peptidases in the simulated digestion of milk proteins
Author(s) -
Picariello Gianluca,
Miralles Beatriz,
Mamone Gianfranco,
SánchezRivera Laura,
Recio Isidra,
Addeo Francesco,
Ferranti Pasquale
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400856
Subject(s) - digestion (alchemy) , brush border , bioavailability , chemistry , proteases , hydrolysis , skimmed milk , food science , chromatography , biochemistry , biology , enzyme , vesicle , membrane , pharmacology
Scope This study aimed to assess the impact of the “often neglected” intestinal brush border membranes (BBMs) hydrolases on dietary peptides, exploring the possibility that the disintegration of proteins progressed in the small intestine up to a “core” of intrinsically stable oligopeptides, persisting independently on the up‐stream breakdown. Methods and results Samples of sodium caseinate, skim milk powder, and whey protein isolate were submitted to in vitro simulated gastropancreatic digestion using two different procedures: (i) a simplified model involving the main compartmental specific proteases; (ii) a static digestion method based on a frameset of parameters inferred from in vivo. The gastroduodenal digesta were further hydrolyzed with peptidases from porcine jejunal BBM. The peptidomes arising from the two digestion models, characterized by combined HPLC and MS techniques, differed to some extent. However, only specific protein domains survived digestion, among which are potential bioactive or immunogenic (food allergy) peptides. The degree of hydrolysis (DH) after BBM digestion (70–77%) practically did not differ between the digestion models and significantly increased the DH after duodenal steps. Conclusion Any in vitro digestion model should be supplemented with a jejunal phase to realistically determine the bioaccessibility and bioavailability of dietary peptides.