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Quercetin and isorhamnetin aglycones are the main metabolites of dietary quercetin in cerebrospinal fluid
Author(s) -
Wiczkowski Wiesław,
Skipor Janina,
Misztal Tomasz,
SzawaraNowak Dorota,
Topolska Joanna,
Piskula Mariusz K.
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400567
Subject(s) - isorhamnetin , quercetin , cerebrospinal fluid , urine , chemistry , in vivo , blood–brain barrier , pharmacology , blood plasma , biochemistry , endocrinology , medicine , biology , central nervous system , kaempferol , antioxidant , microbiology and biotechnology
Scope Reports on the protective effect of certain foods on brain functions are numerous; however, the permeability of the brain barriers by food components is still hardly recognised. There have been in vitro studies aimed at demonstrating this possibility, but not much is known about this phenomenon in in vivo systems. The objective of the study was to determine the metabolites of dietary quercetin (Q) in urine, blood plasma and cerebrospinal fluid (CSF) after intra‐rumen administration of Q rich onion dry skin in an animal model. Methods and results Eleven sheep had permanently implanted cannulas in the third ventricle of the brain as the means for CSF collection. The animals were administered Q at the dose of 10 mg/kg bwt. For 12 h the concentration of Q metabolites was measured in urine, blood plasma, and CSF. It was demonstrated that while in blood plasma Q and isorhamnetin mono‐glucuronides or mono‐sulphates were the main metabolites (80%), in CSF their aglycones were the dominating ones (88%). Conclusion Q and IR aglycones are the main Q metabolites present in CSF after dietary Q intake. Their passive transport through blood–CSF barrier or a de‐conjugating mechanism within that barrier may be involved.