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Green tea changes serum and liver metabolomic profiles in mice with high‐fat diet‐induced obesity
Author(s) -
Lee LanSook,
Choi Ji Hea,
Sung Mi Jeong,
Hur JinYoung,
Hur Haeng Jeon,
Park JongDae,
Kim YoungChan,
Gu EunJi,
Min Byungjin,
Kim HyunJin
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400470
Subject(s) - endocrinology , medicine , metabolomics , leptin , lipid metabolism , obesity , diet induced obese , carnitine , adipose tissue , chemistry , coenzyme a , fenofibrate , biochemistry , insulin resistance , enzyme , chromatography , reductase
Scope Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT‐induced changes in serum and liver metabolomes associated with the anti‐obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT‐fed obese mice to elucidate the relationship between GT consumption and obesity prevention. Methods and results Serum and liver metabolites were analyzed in mice fed normal diet, high‐fat diet (HFD), HFD with GT, and HFD with crude catechins, using LC‐quadrupole TOF MS. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD‐fed mice. We proposed an HFD‐induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β‐oxidation: carnitine palmitoyltransferase‐1 and ‐2, acyl‐coenzyme A dehydrogenase, and acetyl‐coenzyme A acyltransferase. The results showed that HFD‐induced abnormal mitochondrial β‐oxidation was moderated by the consumption of caffeine‐ and theanine‐enriched GT. Conclusion Results of LC/MS‐based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti‐obesity effects of GT.