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Molecular mechanism driving retroperitoneal adipocyte hypertrophy and hyperplasia in response to a high‐sugar diet
Author(s) -
de Queiroz Karina Barbosa,
Coimbra Roney Santos,
Ferreira Amanda Rios,
Carneiro Cláudia Martins,
Paiva Nívia Caroliogueira,
Costa Daniela Caldeira,
Evangelista Elísio Alberto,
GuerraSá Renata
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400241
Subject(s) - adipocyte , adipogenesis , endocrinology , medicine , biology , adipose tissue , hyperplasia , white adipose tissue
Scope We have previously shown an increase in adipocyte size and lipid content in retroperitoneal white adipose tissue (rWAT) induced by an 8‐week high‐sugar diet (HSD). In this study, we assessed the effect of a HSD on the transcriptional activity of adipogenic genes in a time‐course study to provide insight regarding the genetic networks involved in the rWAT response to dietary sugar. Methods and results Weaned male Wistar rats were fed a standard chow diet or HSD (68% carbohydrates) for 4, 8 or 12 weeks, and rWAT was removed for histopathology and PCR array (adipogenesis) analyses. The HSD induced adipocyte hypertrophy and hyperplasia in rWAT after 12 weeks of ingestion. Additionally, the HSD altered serum VLDL‐cholesterol, triacylglycerol and glucometabolic parameters. Hierarchical clustering revealed HSD‐induced changes in the expression patterns of the tested gene set. Pathway analysis, which used the enrichment analysis algorithm of the Thompson Reuters MetaCore platform, associated a cluster of differentially expressed genes with canonical pathways related to regulating adipocyte differentiation and proliferation ( p ‐value < 10 −7 ). Conclusion HSD feeding post‐weaning increased both the adipocyte size and number by simultaneously up‐regulating pro‐adipogenic signals (the PPARγ pathway) and down‐regulating anti‐adipogenic signals (Wnt pathway) in young adults.