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Isothiocyanate metabolism, distribution, and interconversion in mice following consumption of thermally processed broccoli sprouts or purified sulforaphane
Author(s) -
Bricker Gregory V.,
Riedl Kenneth M.,
Ralston Robin A.,
Tober Kathleen L.,
Oberyszyn Tatiana M.,
Schwartz Steven J.
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400104
Subject(s) - sulforaphane , metabolite , chemistry , glucoraphanin , glucosinolate , myrosinase , isothiocyanate , metabolism , food science , biochemistry , botany , biology , brassica
Scope Broccoli sprouts are a rich source of glucosinolates, a group of phytochemicals that when hydrolyzed, are associated with cancer prevention. Our objectives were to investigate the metabolism, distribution, and interconversion of isothiocyanates (ITCs) in mice fed thermally processed broccoli sprout powders (BSPs) or the purified ITC sulforaphane. Methods and results For 1 wk, mice were fed a control diet ( n = 20) or one of four treatment diets ( n = 10 each) containing nonheated BSP, 60°C mildly heated BSP, 5‐min steamed BSP, or 3 mmol purified sulforaphane. Sulforaphane and erucin metabolite concentrations in skin, liver, kidney, bladder, lung, and plasma were quantified using HPLC‐MS/MS. Thermal intensity of BSP processing had disparate effects on ITC metabolite concentrations upon consumption. Mild heating generally resulted in the greatest ITC metabolite concentrations in vivo, followed by the nonheated and steamed BSP diets. We observed interconversion between sulforaphane and erucin species or metabolites, and report that erucin is the favored form in liver, kidney, and bladder, even when only sulforaphane is consumed. Conclusion ITC metabolites were distributed to all tissues analyzed, suggesting the potential for systemic benefits. We report for the first time tissue‐dependent ratio of sulforaphane and erucin, though further investigation is warranted to assess biological activity of individual forms.