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Modulation of human miR‐17–3p expression by methyl 3‐ O ‐methyl gallate as explanation of its in vivo protective activities
Author(s) -
Curti Valeria,
Capelli Enrica,
Boschi Federica,
Nabavi Seyed Fazel,
Bongiorno Andria I.,
Habtemariam Solomon,
Nabavi Seyed Mohammad,
Daglia Maria
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201400007
Subject(s) - antioxidant , in vivo , propyl gallate , microrna , enzyme , chemistry , methyl gallate , oxidative stress , peripheral blood mononuclear cell , microvesicles , messenger rna , gene expression , mechanism of action , gene silencing , ex vivo , microbiology and biotechnology , in vitro , biochemistry , biology , gene , genetics , gallic acid
Scope Methyl‐3‐ O ‐methyl gallate (M3OMG) possesses in vivo antioxidant activity due to the partial restoration of the antioxidant enzymes, whose expression is altered in oxidative stress. Literature data suggest that miR‐17–3p is a microRNA involved in the regulation of cellular redox status, interfering with transcription of the mRNAs responsible for the synthesis of antioxidant enzymes. To obtain deeper insight into the potential mechanism of action of M3OMG, the aim of this study was to investigate its effect on the expression levels of miR‐17–3p in human cells. Methods and results Peripheral blood mononuclear cells and EVC‐304 cells were treated with increasing subtoxic concentrations of M3OMG. The expression levels of miR‐17–3p, extracted from cells and exosomes, were determined by quantitative real‐time PCR. M3OMG induced a decrease in the miR‐17–3p levels, and an increase in the levels of mRNA coding for the antioxidant enzymes, when compared to the control samples. Differently, in exosomes the expression levels of miR‐17–3p were depended on the compound, its concentration, and the type of cell. Conclusion These results suggest a potential mechanism of action of M3OMG that, inducing the reduction of the levels of miR‐17–3p and the increase of mRNA coding for antioxidant enzymes, allows to these latter to perform their protective effects.