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Glycyrrhizic acid attenuated glycative stress in kidney of diabetic mice through enhancing glyoxalase pathway
Author(s) -
Wang Zhihong,
Hsieh ChengHong,
Liu Wenhu,
Yin Meichin
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300910
Subject(s) - methylglyoxal , medicine , endocrinology , chemistry , aldose reductase , lactoylglutathione lyase , kidney , renal function , oxidative stress , glutathione , diabetes mellitus , biochemistry , enzyme
Scope Antiglycative effects of glycyrrhizic acid (GA) in kidney of diabetic mice were examined. Methods and results GA at 0.05, 0.1, and 0.2% was supplied to diabetic mice for 9 wk. Results showed that GA intake increased its deposit in kidney, raised plasma insulin level, decreased plasma glucose and blood urine nitrogen levels, and improved creatinine clearance rate ( p < 0.05). GA intake dose‐dependently reduced renal carboxymethyllysine level, and at 0.1 and 0.2% decreased plasma HbA1c, urinary glycated albumin, and renal pentosidine levels ( p < 0.05). Dietary GA intake declined renal aldose reductase activity and protein expression, as well as lowered renal fructose and sorbitol levels ( p < 0.05). GA intake dose‐dependently increased glyoxalase‐1 activity and expression, and decreased renal methylglyoxal level ( p < 0.05). This compound at 0.1 and 0.2% raised glyoxalase‐2 activity and protein expression, and increased d ‐lactate formation ( p < 0.05). GA intake dose‐dependently suppressed renal expression of nuclear factor kappa B (NF‐κB) p65 and p‐p38, decreased reactive oxygen species production, and retained glutathione content ( p < 0.05). This compound at 0.1 and 0.2% downregulated renal expression of NF‐κB p50 and p‐ERK1/2 ( p < 0.05), and lowered renal level of monocyte chemoattractant protein‐1 (MCP‐1) and intercellular adhesion molecule‐1 (ICAM‐1). Conclusions These findings suggest that glycyrrhizic acid is an antiglycative and renal‐protective agent.