Premium
Resveratrol inhibits glucose‐induced migration of vascular smooth muscle cells mediated by focal adhesion kinase
Author(s) -
Lin YiChiao,
Chen LiHsuen,
Varadharajan T.,
Tsai MayJywan,
Chia YiChen,
Yuan TaChun,
Sung PingJyun,
Weng ChingFeng
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300698
Subject(s) - resveratrol , lamellipodium , vascular smooth muscle , rac1 , chemistry , microbiology and biotechnology , cell migration , cdc42 , protein kinase a , focal adhesion , proto oncogene tyrosine protein kinase src , kinase , endocrinology , signal transduction , biology , biochemistry , cell , smooth muscle
Scope Diabetes is a critical factor for atherosclerosis, as hyperglycemia induces vascular smooth muscle cell ( VSMC ) proliferation and migration and subsequently contributes to the formation of atherosclerotic lesions. This study investigates whether resveratrol plays a regulatory role in the proliferation and migration of VSMC s under high glucose induction to imitate a hyperglycemic condition. Methods and results Resveratrol inhibited the migration of VSMC s in the wound‐healing assay and the formation of lamellipodia and filopodia as assessed by atomic force microscopy scanning. Resveratrol suppressed the m RNA expression of c‐Src, Rac1, cdc42, IRS ‐1, MEKK 1, MEKK 4, and mitogen‐activated protein kinase along with the protein levels of c‐Src, p‐Src, and cdc42 in VSMC s. Resveratrol decreased the level of p‐ FAK protein under normal glucose conditions. Resveratrol could inhibit the activities of matrix metalloproteinase ( MMP ) 2 and MMP 9 as shown by zymography. Moreover, resveratrol also regulated the mitogen‐activated protein kinase pathway and MMP activities of VSMC migration under the high glucose condition. Conclusion The antimigratory effects of resveratrol by reduced MMP expression through the inhibition of R ac1, p‐ FAK , and lamellipodia formation and the activation of p‐ AKT and p‐ ERK 1/2 suggest that resveratrol is a potential compound for the treatment of vascular diseases via the regulation of VSMC migration.