Premium
Identification of S ola l 4 as B et v 1 homologous pathogenesis related‐10 allergen in tomato fruits
Author(s) -
Wangorsch Andrea,
Jamin Annette,
Foetisch Kay,
Malczyk Anna,
Reuter Andreas,
Vierecke Sabine,
Schülke Stefan,
Bartel Detlef,
Mahler Vera,
Lidholm Jonas,
Vieths Stefan,
Scheurer Stephan
Publication year - 2015
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300620
Subject(s) - allergen , pollen , immunoglobulin e , allergy , immunology , pathogenesis , cross reactivity , recombinant dna , biology , botany , antibody , cross reactions , biochemistry , gene
Scope The aim was to investigate the potential contribution of a major birch pollen B et v 1‐homologous allergen to birch pollen‐associated tomato fruit allergy. Methods and results Two isoforms of a B et v 1‐homologous protein (designated S ola l 4) from tomato fruit were identified by c DNA ‐cloning and produced as recombinant proteins. Allergen‐specific I g E levels to tomato, birch pollen, B et v 1, and S ola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato‐ and B et v 1‐specific I g E was detectable in 64% and 81% of sera. Almost all patients sensitized to B et v 1 reacted with S ola l 4. Both Sola l 4 isoforms displayed allergenic potency and I g E ‐cross‐reactivity with B et v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse. Conclusion Sola l 4, a novel pathogenesis related‐10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. Ig E ‐testing using S ola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component‐resolved allergy diagnosis.