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Diallyl sulfide as a potential dietary agent to reduce TNF ‐α‐ and histamine‐induced proinflammatory responses in A 7r5 cells
Author(s) -
Ho ChengYing,
Weng ChiaJui,
Jhang JhihJia,
Cheng YuTing,
Huang ShangMing,
Yen GowChin
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300617
Subject(s) - oxidative stress , tumor necrosis factor alpha , proinflammatory cytokine , histamine , inflammation , chemistry , pharmacology , reactive oxygen species , cytokine , endocrinology , immunology , biochemistry , medicine
Scope Oxidative stress‐aggravated chronic inflammatory diseases of the airway are well documented; hence, treatment with antioxidants to ameliorate oxidative stress might be an effective strategy to reduce airway complications. The aim of this study was to investigate the effect and molecular mechanism of diallyl sulfide ( DAS ), which is a natural organosulfuric compound found in garlic, on the inhibition of tumor necrosis factor‐alpha ( TNF ‐α)‐ or histamine‐induced inflammation in rat aortic smooth muscle A 7r5 cells. Methods and results A 7r5 cells were coincubated with DAS before exposure to TNF ‐α or histamine. DAS significantly blocked the accumulation of the nuclear p65 protein in TNF ‐α‐induced A 7r5 cells by attenuating the TNF ‐α receptor complex through the dissociation of the TNF receptor‐associated death domain and TNF receptor‐associated factor 2. Moreover, DAS inhibited histamine‐induced inflammation by decreasing reactive oxygen species ( ROS ) levels by enhancing the nuclear factor‐erythroid 2‐related factor 2‐related antioxidative enzyme. DAS also inhibited inflammation by suppressing interleukin‐1β and TNF ‐α through the inhibition of ROS ‐induced PI 3 K / A kt and the downstream NF ‐κB and activator protein‐1. Conclusion Our results demonstrate that DAS is a potential phytochemical to inhibit TNF ‐α‐ and histamine‐induced inflammation, suggesting that DAS might be an effective dietary agent for the prevention of oxidative stress‐induced inflammation of the airway.