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Selenite protects C aenorhabditis elegans from oxidative stress via DAF ‐16 and TRXR ‐1
Author(s) -
Li WenHsuan,
Shi YeuChing,
Chang ChunHan,
Huang ChiWei,
HsiuChuan Liao Vivian
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300404
Subject(s) - caenorhabditis elegans , oxidative stress , selenoprotein , selenium , rna interference , superoxide dismutase , downregulation and upregulation , biology , chemistry , microbiology and biotechnology , mutant , biochemistry , rna , gene , glutathione peroxidase , organic chemistry
Scope Selenium is an essential micronutrient. In the present study, trace amount of selenite (0.01 μM) was evaluated for oxidative stress resistance and potential associated factors in C aenorhabditis elegans . Methods and results Selenite‐treated C . elegans showed an increased survival under oxidative stress and thermal stress compared to untreated controls. Further studies demonstrated that the significant stress resistance of selenite on C . elegans could be attributed to its in vivo free radical‐scavenging ability. We also found that the oxidative and thermal stress resistance phenotypes by selenite were absent from the forkhead transcription factor daf‐16 mutant worms. Moreover, selenite influenced the subcellular distribution of DAF ‐16 in C . elegans . Furthermore, selenite increased m RNA levels of stress‐resistance‐related proteins, including superoxide dismutase‐3 and heat shock protein‐16.2. Additionally, selenite (0.01 μM) upregulated expressions of transgenic C . elegans carrying sod‐3 ::green fluorescent protein ( GFP ) and hsp‐16.2 :: GFP , whereas this effect was abolished by feeding daf‐16 RNA interference in C . elegans . Finally, unlike the wild‐type N 2 worms, the oxidative stress resistance phenotypes by selenite were both absent from the C . elegans selenoprotein trxr‐1 mutant worms and trxr‐1 mutants feeding with daf‐16 RNA interference. Conclusion These findings suggest that the antioxidant effects of selenite in C . elegans are mediated via DAF ‐16 and TRXR ‐1.