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Characterization of I le‐ H is‐ A rg‐ P he, a novel rice‐derived vasorelaxing peptide with hypotensive and anorexigenic activities
Author(s) -
Kontani Noriyasu,
Omae Ryo,
Kagebayashi Tomomi,
Kaneko Kentaro,
Yamada Yuko,
Mizushige Takafumi,
Kanamoto Ryuhei,
Ohinata Kousaku
Publication year - 2014
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300334
Subject(s) - chemistry , cholecystokinin , peptide yy , peptide , glutelin , biochemistry , receptor , pharmacology , storage protein , neuropeptide , biology , neuropeptide y receptor , gene
Scope Recently, we found that dipeptide A rg‐ P he ( RF ) had cholecystokinin ( CCK )‐dependent vasorelaxing activity. The RF sequence is often observed in the primary structure of natural food proteins. In the current study, we investigated enzymatic conditions for the release of RF ‐related peptides from rice glutelin, a major storage protein, using gastrointestinal proteases. RF ‐related peptides were then characterized. Methods and results It was found that RF and I le‐ H is‐ A rg‐ P he ( IHRF ) were released in the chymotrypsin digest of the partial structure of rice glutelin. We then focused on previously unidentified IHRF , corresponding to rice glutelin(155−158). IHRF had vasorelaxing activity in the mesenteric artery of spontaneous hypertensive rats ( SHR s). Orally administered IHRF lowered systolic blood pressure in SHR s. The antihypertensive activity of IHRF was more potent and long‐lasting than that of RF . IHRF ‐induced vasorelaxing activity was not blocked by inhibitors of nitric oxide synthase and cyclooxygenase, but by an antagonist for CCK 1 receptor. IHRF also had CCK ‐like suppressive activities in food intake and gastrointestinal transit. IHRF increased intracellular C a 2+ flux and CCK release in the enteroendocrine cell STC ‐1. Conclusion IHRF , a novel CCK ‐dependent vasorelaxing peptide, decreases both blood pressure and food intake in rodents.