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Curcumin protects neuronal‐like cells against acrolein by restoring A kt and redox signaling pathways
Author(s) -
Doggui Sihem,
Belkacemi Abdenour,
Paka Ghislain Djiokeng,
Perrotte Morgane,
Pi Rongbiao,
Ramassamy Charles
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300130
Subject(s) - curcumin , acrolein , chemistry , neuroprotection , glutathione , reactive oxygen species , toxicity , antioxidant , biochemistry , pharmacology , biology , enzyme , organic chemistry , catalysis
Scope The aim of the present study was to examine the neuroprotective effect of curcumin against the toxicity induced by acrolein and to identify its cellular mechanisms and targets. Methods and results Human neuroblastoma cells SK‐N‐SH were treated with acrolein. Curcumin, from 5 μM, was able to protect SK‐N‐SH cells against acrolein toxicity. The addition of curcumin restored the expression of γ‐glutamylcysteine synthetase, reactive oxygen species, and reactive nitrogen species levels but had no effect on the decrease of glutathione ( GSH ) and on the elevation of protein carbonyls. Acrolein induced the activity of N rf2, NF ‐κ B , and S irt1. These activations were prevented by the presence of curcumin. Acrolein also induced a decrease of the p A kt, which was counteracted by curcumin. To increase its solubility, we have encapsulated curcumin in a biodegradable poly(lactide‐co‐glycolide) based nanoparticulate formulation ( N ps‐ C ur). Our results showed that 0.5 μM of N ps‐ C ur can protect neuronal cells challenged with acrolein while free curcumin was not able to display neuroprotection. Conclusion Our results provided evidence that curcumin was able to protect SK‐N‐SH cells against acrolein toxicity. This protection is mediated through the antioxidant, the redox, and the survival regulated pathways by curcumin. Moreover, our results demonstrated that Nps‐Cur had higher capacity than curcumin to protect SK‐N‐SH cells against acrolein.