Premium
Role of anthocyanin‐enriched purple‐fleshed sweet potato p40 in colorectal cancer prevention
Author(s) -
Lim Soyoung,
Xu Jianteng,
Kim Jaeyong,
Chen TzuYu,
Su Xiaoyu,
Standard Joseph,
Carey Edward,
Griffin Jason,
Herndon Betty,
Katz Benjamin,
Tomich John,
Wang Weiqun
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300040
Subject(s) - azoxymethane , anthocyanin , apoptosis , in vivo , chemistry , aberrant crypt foci , colorectal cancer , cell cycle , food science , cancer , biology , biochemistry , microbiology and biotechnology , colonic disease , genetics
Scope Anthocyanins, the natural pigments in plant foods, have been associated with cancer prevention. However, the content of anthocyanins in staple foods is typically low and the mechanisms by which they exert anticancer activity is not yet fully defined. Methods and results We selected an anthocyanin‐enriched purple‐fleshed sweet potato clone, P 40, and investigated its potential anticancer effect in both in vitro cell culture and in vivo animal model. In addition to a high level of total phenolics and antioxidant capacity, P 40 possesses a high content of anthocyanins at 7.5 mg/g dry matter. Treatment of human colonic SW 480 cancer cells with P 40 anthocyanin extracts at 0–40 μM of peonidin‐3‐glucoside equivalent resulted in a dose‐dependent decrease in cell number due to cytostatic arrest of cell cycle at G 1 phase but not cytotoxicity. Furthermore, dietary P 40 at 10–30% significantly suppressed azoxymethane‐induced formation of aberrant crypt foci in the colons of CF ‐1 mice in conjunction with, at least in part, a lesser proliferative PCNA and a greater apoptotic caspase‐3 expression in the colon mucosal epithelial cells. Conclusion These observations, coupled with both in vitro and in vivo studies reported here, suggest anthocyanin‐enriched sweet potato P 40 may protect against colorectal cancer by inducing cell‐cycle arrest, antiproliferative, and apoptotic mechanisms.