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Protective effects of hydroxytyrosol on liver ischemia/reperfusion injury in mice
Author(s) -
Pan Shangha,
Liu Lianxin,
Pan Huayang,
Ma Yong,
Wang Dawei,
Kang Kai,
Wang Jizhou,
Sun Bei,
Sun Xueying,
Jiang Hongchi
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201300010
Subject(s) - hydroxytyrosol , oxidative stress , superoxide dismutase , malondialdehyde , pharmacology , liver injury , catalase , reactive oxygen species , antioxidant , chemistry , reperfusion injury , tumor necrosis factor alpha , apoptosis , necrosis , ischemia , biochemistry , endocrinology , medicine , polyphenol
Scope Hydroxytyrosol ( HT ), a main phenolic compound in olive oil, has been proved to be a potent antioxidant and has beneficial effects on health. However, the effect of HT on oxidative liver damage, as seen in ischemia/reperfusion ( I / R ) injury, is unknown. Here, we examined whether HT could protect liver against I / R injury. Methods and results By using a mouse model, we found that HT administration protects against hepatic I / R injury, as indicated by the decreased levels of serum aminotransferase and less parenchymal necrosis and apoptosis. Serum levels of tumor necrosis factor‐α, interleukin‐6, macrophage inflammatory protein 2, as well as reactive oxygen species (ROS) content in liver tissues, were all decreased by HT , the latter correlated with the reduction of hepatic malondialdehyde (an index of oxidative stress) content and increased activities and expressions of liver antioxidant enzymes superoxide dismutase and catalase. The protective effect was also seen in isolated hepatocytes anoxia/reoxygenation assay. Conclusion HT exerts protective effects against hepatic I / R injury in mice, which might be associated with its anti‐oxidative, anti‐inflammatory, and anti‐apoptotic properties. HT may be an effective hepatoprotective agent and a promising candidate for the treatment of liver I / R injury.