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Deoxynivalenol impairs the immune functions of neutrophils
Author(s) -
Gauthier Thierry,
Waché Yann,
Laffitte Joëlle,
Taranu Ionelia,
Saeedikouzehkonani Nazli,
Mori Yasuyuki,
Oswald Isabelle P.
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200755
Subject(s) - immune system , p38 mitogen activated protein kinases , western blot , phagocytosis , chemotaxis , biology , signal transduction , protein kinase a , kinase , microbiology and biotechnology , flow cytometry , phosphorylation , secretion , immunology , biochemistry , receptor , gene
Scope Deoxynivalenol ( DON ), a mycotoxin produced by F usarium spp., is toxic to many animal species, with pigs being the most sensitive species to the toxin. The aim of the present study was to determine the effects of DON on pig polymorphonuclear cells ( PMN s), the first line of defense against infection. Methods and results PMN s isolated from pig blood samples were stimulated with LPS to mimic infection. DON (0.5–10 μM) altered three main functions of pig PMN s: LPS ‐induced secretion of IL ‐8, chemotaxis, and phagocytosis capability. This alteration of PMN properties was due to apoptotis induced by DON exposure. Using W estern blot and flow cytometry, we demonstrated that this process included the permeabilization of the mitochondrial outer membrane and the activation of caspase‐3. The effect of DON was mediated by the phosphorylation of the p38 mitogen‐activated protein kinase within the first 30 min of exposure. Conclusion This study provides evidence that low concentrations of DON can alter the immune functions of porcine PMN s and suggests the involvement of p38 mitogen‐activated protein kinase in the signal transduction pathway. These immunosuppressive effects of DON may have implications for humans and/or animals when eating contaminated food/feed.