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Autophagy is involved in the effects of resveratrol on prevention of splenocyte apoptosis caused by oxidative stress in restrained mice
Author(s) -
Duan WenJun,
Liu FangLan,
He RongRong,
Yuan WeiLin,
Li YiFang,
Tsoi Bun,
Su WeiWei,
Yao XinSheng,
Kurihara Hiroshi
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200662
Subject(s) - resveratrol , splenocyte , oxidative stress , autophagy , pharmacology , spleen , reactive oxygen species , antioxidant , apoptosis , immunity , chemistry , downregulation and upregulation , immunology , immune system , biology , biochemistry , gene
Scope Resveratrol, a powerful natural compound for human health, is widely reported for its immunity‐related beneficial properties. However, few works have studied its effect mechanism on immunity. The present study was conducted to investigate the effects of resveratrol on splenic immunity in restraint stressed mice and the mechanism was further studied as autophagy induction. Methods and results Mice were administered with resveratrol for 7 days consecutively, fixed in restraint cages for 18 h, and recovered for 12 h after the last administration. Data showed that restraint led to spleen damages, including declined spleen index, decreased CD 4 + T ‐cell number, increased mitochondrial oxidative damage, and apoptosis of splenocytes. Resveratrol, vitamin C (antioxidant), and rapamycin (autophagy agonist) protected spleen functions. Meanwhile, rapamycin augmented the effects of resveratrol that were abolished by chloroquine (autophagy antagonists). Further studies showed that expressions of B eclin 1 and LC 3β required in autophagy development were significantly upregulated by resveratrol but not by vitamin C . Conclusion This study demonstrated that resveratrol preserved splenic immunity of restraint stressed mice. It is meaningful to find that autophagy, apart from reactive oxygen species clearance, is included as a potential mechanism via which resveratrol ameliorated the state of oxidative stress and thus protected splenocytes in mice.